FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217341119
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99237644.56598550962646No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTGCTTATCTCGTAT8695060.40006511607221457TruSeq Adapter, Index 14 (97% over 39bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7630030.3510624236732673No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6157260.2832993603939253No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5534260.25463474309249323No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4969410.2286456434412671No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTGCTTATCGCGTAT3899020.1793963341101598TruSeq Adapter, Index 14 (97% over 39bp)
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3579830.16471020377878887No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3359780.15458556647994437No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2401580.11049818879417843No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)