FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197311321
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64294343.258522606515822No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6492660.32905663836693894No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4518640.22901068104450023No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGAACATCTCGTAT4452620.22566469969556385TruSeq Adapter, Index 3 (97% over 36bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4413720.22369319599254014No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4194910.21260361436635458No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2882520.14608994483393073No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2701930.13693740360696283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)