FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212390696
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA116363655.478754587253672No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTTATCATCTCGTAT8868620.4175616054292698TruSeq Adapter, Index 14 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7641310.35977611749998695No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5515500.25968651658827846No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4994690.23516519763182092No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4974350.23420752856330393No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3312680.15597105063396938No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3187520.15007813713271131No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2343450.11033675411092395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)