FastQCFastQC Report
Wed 15 May 2024
EGAF00006142001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297979617
Total Bases44.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77320362.5948204369965344No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGTCTATATCTCGTAT7511700.25208771242900146TruSeq Adapter, Index 15 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7434480.24949626000760985No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5824760.19547511533314038No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5187030.17407331589395258No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4752900.15950419857073647No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3426060.11497632067900805No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3331550.1118046272272375No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGTCTATATCGCGTAT3163960.10618041703167906TruSeq Adapter, Index 15 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)