FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212290212
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35915551.6918137516391947No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGAGCTATCTCGTAT5067570.23870954540287517TruSeq Adapter, Index 5 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4949460.23314593515032148No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3547570.16710944732581454No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200080.15074081700950018No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3193720.1504412271254409No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGAGCTATCGCGTAT2457600.11576605331196334TruSeq Adapter, Index 5 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)