FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171318224
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60760863.5466664655594373No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGTCTGATCTCGTAT15113010.8821600905692322TruSeq Adapter, Index 12 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7212940.4210258448628326No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5649370.32975884690469354No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGTCTGATCGCGTAT4527780.2642906221115157TruSeq Adapter, Index 12 (97% over 38bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4369040.25502482444599706No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4071520.23765831240463944No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2735640.15968178610116807No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2575740.1503482781843454No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)