FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191164688
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44472712.3264082119601506No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTATAACAATCTCGTAT5442840.2847199478598265TruSeq Adapter, Index 21 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4637590.2425965824818023No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3585910.1875822379915688No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3193960.1670789743344231No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3104750.16241231748825913No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2034290.10641557398927148No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)