FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216882123
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA167094877.70440955154243No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8422740.388355659908401No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7515750.3465361688662555No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6823480.31461698666607024No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCGCAACATCTCGTAT5981970.2758166471839636TruSeq Adapter, Index 6 (97% over 36bp)
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4923740.22702378286844788No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4232900.19517053510214855No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4136170.19071050867571968No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCGCAACATCGCGTAT2536950.11697367975321783TruSeq Adapter, Index 6 (97% over 36bp)
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240860.10332156329915675No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)