FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215429605
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52090802.417996356628886No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5887030.27326931226560064No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTTAGCATCTCGTAT5822660.2702813292537022TruSeq Adapter, Index 7 (97% over 35bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4216540.19572704503635885No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4015030.18637317744699017No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820140.17732660281301635No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTTAGCATCGCGTAT2554810.11859140715594776TruSeq Adapter, Index 7 (97% over 35bp)
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2403460.11156591035851363No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2317110.10755764046450347No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)