FastQCFastQC Report
Mon 20 May 2024
EGAF00006142027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182242607
Total Bases27.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41482292.27621249952817No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5167330.2835412687001344No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3679550.20190393786454122No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3596830.1973649334373273No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTAATCAATCTCGTAT3585930.19676682961410882TruSeq Adapter, Index 22 (97% over 37bp)
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3315170.18190971115772064No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2312780.12690665690488065No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2133000.1170417848555031No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)