FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165607995
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131043187.912853482707765No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTAGATCTCGTAT14490950.87501512230735TruSeq Adapter, Index 5 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7641060.4613943910135498No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5988140.3615852000381986No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5330320.3218636878008215No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5120020.30916502551703495No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTAGATCGCGTAT4569050.2758954964704452TruSeq Adapter, Index 5 (97% over 38bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3590310.2167956927441818No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3517650.21240822340733004No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3351640.2023839489150267No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2960250.17875042808168773No Hit
AAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2474430.1494148878500703No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1778390.10738551601932021No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1738750.10499191177334162No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730020.10446476331049115No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)