FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167711009
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40645742.423558253113843No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAACTGATCTCGTAT8890680.5301190454348766TruSeq Adapter, Index 6 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4943060.29473676352397354No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3560940.21232595410597047No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3372260.2010756491245008No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3199980.19080321674053013No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2053980.1224713876713961No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2047280.12207189093949104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)