FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183835782
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27958041.520816007408177No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCACAGATCTCGTAT8077760.43940085614018276TruSeq Adapter, Index 18 (97% over 40bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4587450.2495406470977451No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3276810.1782465831379878No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200580.17409994752816946No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2836200.15427899667541328No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCACAGATCGCGTAT2699520.14684410024159497TruSeq Adapter, Index 18 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)