FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238151470
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46000671.93157195292559No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAAGATCTCGTAT8645080.3630076270366922TruSeq Adapter, Index 7 (97% over 35bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6180770.2595310455148566No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4456410.18712502593412503No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4254770.17865814559112317No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3729100.1565852186425723No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAAGATCGCGTAT3371560.14157208435454965TruSeq Adapter, Index 7 (97% over 35bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2443070.10258471215819076No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)