FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194751146
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31108521.5973472115024165No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4552100.23373931776504153No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3308190.16986754984229976No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3198340.16422701820712265No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3028660.15551436087569928No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTCCGAATCTCGTAT2285970.11737902687360822TruSeq Adapter, Index 14 (97% over 40bp)
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1962190.10075370750321541No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)