FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189776280
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65750113.464611594241388No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGAATGATCTCGTAT7702020.40584734825658925TruSeq Adapter, Index 10 (97% over 36bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5816930.30651512401866027No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4366100.23006563307068723No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4071200.21452628326364076No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3876080.20424470328957867No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGAATGATCGCGTAT2987110.15740165209266405TruSeq Adapter, Index 10 (97% over 36bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2497360.13159494959011736No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2479470.13065226065133115No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)