FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153050463
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA114907837.50783942417737No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAATGGATCTCGTAT11798190.7708692785855865TruSeq Adapter, Index 1 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6455480.4217876818837197No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4642340.3033208726719108No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4509260.29462570132832594No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4026980.2631145258279944No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAATGGATCGCGTAT3944840.25774766849284214TruSeq Adapter, Index 1 (97% over 37bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2675430.1748070504040226No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2535430.16565974060463967No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2359990.15419685466747005No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2108930.13779311468009084No Hit
AAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1625660.10621725463189223No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)