FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225858748
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36452891.613968479095616No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGCAAGATCTCGTAT7680820.3400718399448491TruSeq Adapter, Index 25 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3946260.1747224774308941No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGCAAGATCGCGTAT3128350.13850913580730556TruSeq Adapter, Index 25 (97% over 37bp)
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2716540.12027605855674008No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2679650.1186427368312517No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2323380.10286871863825262No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)