FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184615477
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29734461.6106157773543548No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTTGAATCTCGTAT4866570.26360574308729273TruSeq Adapter, Index 5 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3745640.20288873180443046No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2857740.15479417253841615No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2659230.14404155291920623No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2467390.1336502247858667No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTTGAATCGCGTAT1919390.10396690630656064TruSeq Adapter, Index 5 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)