FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198829027
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60181673.0268050348604283No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6969490.3505267870168675No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4954910.24920455905062594No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4586210.23066098895107504No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4415510.22207572338016823No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3374430.16971515934642684No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCAGGAATCTCGTAT3129670.15740508552606858TruSeq Adapter, Index 9 (97% over 39bp)
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2852800.14348005636018124No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)