FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195117598
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39415202.020074068357484No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACATCTATCTCGTAT4802190.2461177284480511TruSeq Adapter, Index 23 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4503690.23081926213544307No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3330090.17067092021089764No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3151530.16151951604078274No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2973380.15238912483947245No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)