FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200453029
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62825913.134196091394558No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6551580.3268386630366159No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAAGTATCTCGTAT5411370.269957008232587TruSeq Adapter, Index 7 (97% over 35bp)
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540740.22652389054195832No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4452360.22211487759558873No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150750.20706845991336956No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2729240.13615359237100877No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2624930.13094987953512044No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAAGTATCGCGTAT2278680.11367650623029497TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)