FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188605198
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50127232.657786239804483No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTATGTATCTCGTAT4841600.25670554424486225TruSeq Adapter, Index 11 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4802190.2546159941996933No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3525770.18693917439115332No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3234760.17150958904112493No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3135890.16626742174942602No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2168310.11496554829840903No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1976510.1047961573148159No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTATGTATCGCGTAT1975790.10475798233302137TruSeq Adapter, Index 11 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)