FastQCFastQC Report
Sun 19 May 2024
EGAF00006142099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197925579
Total Bases29.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51920712.623244062860617No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5917440.298972979131717No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCAAGGATCTCGTAT5355380.2705754368413393TruSeq Adapter, Index 10 (97% over 37bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4195930.21199533790425337No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3953780.19976094145971907No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810300.19251175210658347No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2508110.12671985160644647No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2395550.12103286559035403No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCAAGGATCGCGTAT2097030.10595042897411455TruSeq Adapter, Index 10 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)