FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224831816
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65234192.9014661341346812No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGATATCTCGTAT6420310.28556056319004247TruSeq Adapter, Index 4 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5953140.26478192036664416No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4471220.1988695407770936No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4085050.18169359091063875No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4065010.18080225798647642No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2602360.11574696349915174No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGATATCGCGTAT2296130.10212656023736427TruSeq Adapter, Index 4 (97% over 37bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2251700.10015041643394455No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)