FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225086712
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50044822.223357369936614No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5548760.24651655136354741No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4122210.18313875409935348No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3936180.17487393924879938No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3663350.16275283278383842No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTCATCTCGTAT2918130.12964470332660064TruSeq Adapter, Index 23 (97% over 40bp)
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2522770.11207991700549608No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2487300.1105040798676734No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)