FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213631136
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47464792.2218104948896586No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATAGTCATCTCGTAT5133480.24029643319408273TruSeq Adapter, Index 2 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4213650.1972395072598406No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3397840.15905172174902443No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3033930.14201721981200344No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2601210.12176174544145099No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)