FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196588191
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50042642.5455567674459143No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5063070.2575470059643613No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTCTATCTCGTAT4478240.22779801661636942TruSeq Adapter, Index 27 (97% over 41bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3974070.20215202041306743No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3613840.18382792891155908No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3321010.16893232411910236No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2301770.11708587317943223No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2105080.10708069438413012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)