FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211734322
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36633571.7301668267084256No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACATTATCTCGTAT6024710.284541020231949TruSeq Adapter, Index 2 (97% over 36bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5186810.2449678422943636No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3738380.17655994383376353No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3585240.1693272949862139No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3217610.1519644982262252No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACATTATCGCGTAT2386980.1127346751085542TruSeq Adapter, Index 2 (97% over 36bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2242720.10592141976868541No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)