FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191317284
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68741203.593047034893094No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACATTATCTCGTAT14717170.7692545959412638TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACATTATCGCGTAT6393840.3342008555797813TruSeq Adapter, Index 2 (97% over 36bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5205040.2720632392000714No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540680.18506848550076638No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3359020.1755732639399167No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2985140.15603085814243528No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2034080.10631971965481175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)