FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205038057
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69191703.3745784081440062No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4806830.23443599058295797No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTCATCTCGTAT4236620.20662603138109137TruSeq Adapter, Index 23 (97% over 40bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3395200.16558877164935287No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3117970.1520678670886937No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3005040.14656010908257877No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2093660.10211079985019561No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)