FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208471629
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59793402.868179247546437No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTGCTGATCTCGTAT18270330.8763940727877173TruSeq Adapter, Index 11 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTGCTGATCGCGTAT6117250.293433213399028TruSeq Adapter, Index 11 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5361370.25717504226918086No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4064600.19497137425831695No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3279010.15728806915976082No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2844030.13642287987302099No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)