FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203459044
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34856431.713191476511607No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGCTAGAATCTCGGTT4191710.2060222990136531TruSeq Adapter, Index 10 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3798810.18671128721119915No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700800.13274416053974972No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2677640.13160584790715912No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2372860.11662592890193664No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)