FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193518797
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34708981.793571505097771No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCTGCCATCTCGTAT5728630.29602447352956623TruSeq Adapter, Index 10 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3878920.20044151059909698No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCTGCCATCGCGTAT2823250.14589022067969967TruSeq Adapter, Index 10 (97% over 38bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2799000.1446371124351295No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2571940.13290388530060984No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2429910.12556454657993765No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)