FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217153859
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42691081.9659369719052517No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGACATCTCGTAT5558550.25597288602640034TruSeq Adapter, Index 25 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4097770.18870353116773303No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2838840.1307294290358432No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2745120.12641359507223862No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGACATCGCGTAT2655690.12229531688865819TruSeq Adapter, Index 25 (97% over 38bp)
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2485540.11445985862033425No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)