FastQCFastQC Report
Fri 6 Jan 2023
EGAF00006142195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195224235
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45032402.3067013170777697No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3802500.19477602255683063No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTATCAAATCTCGTAT3635790.1862366114534909TruSeq Adapter, Index 1 (97% over 37bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3104660.15903046053682834No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2688380.13770728823703676No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2115590.10836718094963979No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)