FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193853096
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36655001.8908648227109046No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCAGGAATCTCGTAT4809410.24809559915411408TruSeq Adapter, Index 9 (97% over 39bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4359570.2248903984489368No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3155970.1628021458063275No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2612900.13478763320860246No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2316630.11950441070077106No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)