FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227616416
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31557811.386447012679437No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAATCATCTCGTAT6108480.26836728683048944TruSeq Adapter, Index 15 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4512200.19823701995202314No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3402360.14947779513407328No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2736750.12023517671062882No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAATCATCGCGTAT2637950.11589454075228037TruSeq Adapter, Index 15 (97% over 37bp)
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2463880.10824702555724276No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)