FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191432329
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25479641.3309998438142598No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCGTTGATCTCGTAT4066440.2124218004995384TruSeq Adapter, Index 18 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3631970.1897260519669068No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2693910.1407238795073114No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2469770.12901530336602654No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2405070.12563551896189906No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)