FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198309729
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22685171.1439262266351038No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATCTAATCTCGTAT4225180.21305964267643168TruSeq Adapter, Index 15 (97% over 36bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4036220.20353111369538507No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3131400.15790450704513845No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2876880.14507003839433413No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2608430.1315331332029605No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)