FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229053400
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37645721.6435346517449643No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5181190.22620009133241417No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3717340.1622914132686963No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3618800.1579893596864312No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCGCAATCTCGTAT3494670.15257009937420707TruSeq Adapter, Index 16 (97% over 38bp)
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3411230.14892728071270717No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)