FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191844426
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37630701.9615216759021188No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTATGCCATCTCGTAT5544260.28899771109325845TruSeq Adapter, Index 22 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5495150.28643782436504045No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4103940.21392021053559304No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3705500.19315129854228863No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3316530.1728760156940916No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTATGCCATCGCGTAT2630380.13711005604092974TruSeq Adapter, Index 22 (97% over 37bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2339020.12192275005164861No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2122900.11065737192698004No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)