FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006142283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006142283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174501661
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27352561.567467028293788No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAACTCGATCTCGTAT7160420.4103353491861605TruSeq Adapter, Index 2 (97% over 36bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3503980.20079923479926076No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAACTCGATCGCGTAT2729730.1564300296259071TruSeq Adapter, Index 2 (97% over 36bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2648570.15177907103130667No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2581940.14796076926740542No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2273000.13025663979209917No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)