FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006143486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1278733
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC696945.450238634648516TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC441603.4534183445645024TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTGC28200.2205307910251788TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTTTGC16530.12926858069667396TruSeq Adapter, Index 3 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATTC15680.12262137600265262TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATGC15650.12238676877815775TruSeq Adapter, Index 3 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)