FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006143492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2984871
Sequences flagged as poor quality0
Sequence length35-75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1885006.315180790057593TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA37240.12476251067466566TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA33970.11380726336247028TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA32180.10781035428331744TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA31870.10677178343720718TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)