FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006143494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2898549
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC187520.646944384931909TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC124560.4297322556906922TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTGC33290.11485056833608816TruSeq Adapter, Index 3 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)