FastQCFastQC Report
Sun 5 Jun 2022
EGAF00006143504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4953837
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC498981.007259625215767TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGC265510.5359683816807053TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTTTGC107230.21645847451177747TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTTTGC57070.11520362902533936TruSeq Adapter, Index 12 (97% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)