FastQCFastQC Report
Mon 6 Jun 2022
EGAF00006143506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544291
Sequences flagged as poor quality0
Sequence length150
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTGG111800.7239568190192134TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGC86510.5601923471677294TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC85050.5507381704613962TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGTTGG81310.5265199369807892TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGG76210.4934950731435979TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTGC69830.45218161602962137TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGTTGC61820.4003131534147385TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGGTGG45840.29683524672487244TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTTTGC44900.29074831103723325TruSeq Adapter, Index 4 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTTG42110.27268176787924037TruSeq Adapter, Index 4 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTTG40990.26542924876205326TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTTTGG34380.22262643504365434TruSeq Adapter, Index 4 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATGC31910.20663204020485776TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGGTGG30970.2005451045172186TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTATGC29840.1932278307650566TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTTC29090.1883712331419402TruSeq Adapter, Index 4 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGATGC28970.18759417752224158TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGGTGC25220.16331118940665976TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGG19260.12471742696162835TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATTC17660.11435668536564675TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTTC16670.10794597650313316TruSeq Adapter, Index 4 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)