FastQCFastQC Report
Sun 5 Jun 2022
EGAF00006143510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3857556
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1767304.581398170240432TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC935152.424203303853528TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCGATGTATCTCGTATGC45610.11823548381410405TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCGATGTATCTCGTATGC43940.11390631788624715TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCGATGTATCTCGTATGC40420.10478136934369843TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCGATGTATCTCGTATGC39970.10361482762661126TruSeq Adapter, Index 2 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)