FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006143524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861602
Sequences flagged as poor quality0
Sequence length35-75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA32919217.683264199329397TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA110770.5950251450095133TruSeq Adapter, Index 16 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78590.4221632765757664No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA63730.342339554856516TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA62490.3356786251841156TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA60460.3247740387042988TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA57840.31070013891261394TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA57570.30924977519362357TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA52550.2822837534553573TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA52300.2809408240859217TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA51090.2744410459378535TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA50490.2712180154512081TruSeq Adapter, Index 16 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA47720.256338358037862TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA47000.25247072145388755TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA41740.22421548752096315TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA41140.22099245703431775TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA39220.21067875947705256TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTTCTTGAAAAAAAAA36570.1964437081610355TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA35210.18913817239130598TruSeq Adapter, Index 16 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA34400.18478708123433474TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCTCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA34050.18290698011712495TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA27860.14965604892990014TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTTAAAAAAAAA27460.14750736193880326TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA27360.146970190191029TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCCCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA24180.12988812861180854TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA23420.1258056233287244TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA22170.11909097648154655TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAA18830.10114944010588729TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)